There are many types of PCR, including routine, Hot Start, Quantitiative PCR, and Long and Accurate. The PCR reagents used in these processes must provide for specific, high yield amplification of DNA. One type is bacterial profiling, which can be used on a variety of materials containing bacteria. Substances tested by bacterial profiling include food for livestock and humans and environmental samples. Bacterial profiling is also used in diagnosing diseases, in forensic soil comparisons, and hospital hygiene applications. Clearly, the PCR reagents used for bacterial profiling must be pure and free of contaminants so that the technique will yield accurate results.
As an example, one of the most important reagents used in this analyses is called DNA polymerase. This substance "reads" a strand of DNA and uses it as a template to create a new strand, copying a piece of DNA. This polymerase uses a magnesium ion for its catalytic activity. There are, however, many other reagents that are used in PCR laboratory studies.
Welcome to PCR reagents
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Polymerase chain reaction, or PCR technology was one of the most important scientific advances in 20th century molecular biology. The technique is used in medical, biological, and even legal applications because it gives such precise and reliable analyses. But it is important for researchers to keep the possibility of contamination with extraneous genetic material at an absolute minimum, because this can lead to wrong results. Researchers minimize these risks by using certified PCR-grade reagents that have been assayed to ensure that there is no contamination of DNase or RNase.