Taq DNA polymerase

Taq DNA polymerase is the standard in polymerase chain reaction (PCR) reagents. Today there are new generation versions of Taq DNA polymerase that are more shelf-stable than older versions. Because they have a higher shelf stability (and can be stored for more than six months at 4 degrees Centigrade), the half-life of active enzymes at high temperatures is increased, allowing more amplification cycles to be carried out, and thus higher yields of replicated DNA. Since these newer reagents don't have to be frozen and thawed, there's less waiting for the reagent to be ready to be used.

But Taq DNA polymerase isn't the only agent used in PCR. There are also PCR Buffers, which are designed to help in purification and analysis of amplified nucleic acid after it has fully replicated. Other necessary solutions for PCR experiments include DNA binding proteins, hot start reagents, real-time PCR buffers, PCR enhancers, mineral oil, and PCR wax.

Many current protocols for PCR amplification call for dispensing and mixing of aqueous solutions in that are refrigerated. Pipetting of enzymes, buffers, and nucleotides can be very time consuming, and the more steps there are, the greater the chance of error. To this end, some science supply companies sell kits of reagents for use in PCR analysis. These include PCR-grade nucleotides, enzyme blends and thermostable enzymes, buffers, and PCR-enhancing agents in one kit. Kits with stabilized PCR subsets that contain reaction buffer plus Taq DNA polymerase is another new approach to the use of PCR reagents. These products help eliminate pipetting errors and cross contamination. The fact that they are stored at room temperature make them even more convenient.